Analysis of regenerating hepatic cell replication in vivo by differential chromatid staining.
CELL AND TISSUE KINETICS(1982)
摘要
The cell cycle of mouse hepatic cells was examined in vivo following partial hepatectomy, by differential chromatid staining in the presence of non-inhibitory concentrations of bromodeoxyuridine (BrdU). Using this technique, distribution curves were obtained for the appearance of metaphase cells in successive generations, and mean cell cycle time (11 hr) was determined. Cell cycle times derived with this technique are several-fold faster than previous reports of regenerating liver which used radionucleotide labelling.
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关键词
Cell Cycle,Hepatic Stellate Cells,Liver Regeneration
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