Chrome Extension
WeChat Mini Program
Use on ChatGLM
VIPCreated with Sketch.

PCR-DGGE技术用于猪场沼气池细菌群落分析的条件优化

WEI Yong,WANG Hong-ning,LIAO Dang-jin,WU Zhi-wei,GUAN Zhong-bin

Journal of Agro-Environment Science（2011）

Cited 22|Views26
No score
Abstract
本研究采用变性梯度凝胶电泳(PCR-DGGE)技术研究猪场沼气池细菌群落,对基于16S rDNA V3区的PCR-DGGE电泳的最佳变性剂梯度范围、电泳时间、染色时间进行优化.研究结果表明,最佳变性剂梯度范围为35%～60%,电泳时间为12 h,SYBR(R)Green Fluorescent Dye染料的染色时间是30 min,优化后的PCR-DGGE确保了实验的准确性、灵敏度和可重复性.运用此优化后的PCR-DGGE技术对5个猪场沼液细菌群落进行了研究,获得了较丰富的多样性.该实验为深入研究畜禽养殖粪污治理的菌种筛选奠定了基础.
More
Translated text
Key words
biogas slurry,pig farm,PCR-DGGE,bacteria communities
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined