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Analysis of biomineral formation in three-dimensional micro-mass stem cell cultures

L. Lammers,U. Meyer,J. Handschel,C. Naujoks,H. P. Wiesmann

EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany（2008）

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Abstract
In our working group a new technique for an improved three-dimensional (3D) cell culture has been developed. Cells self-assemble into a spheroidal cell-agglomerated micro-mass culture (sphere) [1, 2]. These cell-spheres consist of cells and their protein-containing extra-cellular matrix (ECM). In the present study the influence of nutrient-additives on the biomineral formation within the 3D stem cell culture system has been investigated.
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Key words
stem cells, biomineralisation, 3D cell culture
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