A Validated RP-LC Method for the Determination of Isosorbide 5-Mononitrate in a Leishmanicidal Ointment

An RP-LC method using a YMC-Pack ODS-AQ column and UV detection (220 nm) was validated for the determination of isosorbide 5-mononitrate selected as an exogenous NO source in a leishmanicidal ointment. After extraction with hot water, the extracts were analysed at 35 °C using isocratic conditions (water-acetonitrile, 4:1 v/v ; 0.9 mL min −1 ). The method was specific, precise, accurate and linear.