[273] HOX GENES DEREGULATION IN LIVER CARCINOGENESIS

S109the International Working Party (TWP), was proposed standardizing them and establishing diagnostic morphologic criteria for all the hepatocellular lesions, which were classified as: large regenerative nodules (LRN), lowgrade dysplastic nodules (LGDN), high-grade dysplastic nodules (HGDN) and hepatocellular carcinoma (HCC).However, some difficulties in distinguishing between them can still be found.The histological features of these nodules according to IWP and the vascular profile by immunohistochemistry were studied in order to verify its contribution in this aspect.Methods: The histological parameters of 107 nodules of liver explants with morphologic characteristics of LRN, LGDN, HGDN and HCC, according to TWP, from department of pathology ~ UFRJ had been studied.Among these, 90 had been submitted to the immunohistochemistry studies with antibodies (Ah) to CD34 and HHF35 to analyze sinusoidal capillarization and arterialization, respectively.Results: The nodules were classified as follows: 17 LRN, 38 LGDN, 28 HGDN and 24 HCC.The most important histological features that contributed for the diagnosis were: cellularity, thickness of the liver cell plates, cytoplasmic staining, nuclear hyperchromasia, nuclear atypia, pseudoacinar formation, portal tracts, nuclear-cytoplasmic ratio, mitosis/ 1 OHPF and isolated arteries.CD34 antibody demonstrated capillarization over 30% only in the dysplastic and neoplastic nodules, whereas it was over 50% in the HCC, in 85.7% of the cases.The number of isolated arteries, demonstrated with the Ab to HHF35 was very high in HCC (average of 4.032), showing positive correlation when compared with the other nodules (p i 0.005).