RAPD and Microsatellite Analysis of Red Bighead Carp(Aristichthys nobilis)

Genetic variation was comparatively analyzed between the Red bighead carp(RBC) population and the bighead carp(BC) population of aquaculture strains originating from xiangjiang river used as the control,using random amplified polymorphic DNA(RAPD) assay and microsatellite analysis.The specific fragments for RBC and BC population was detected by S20 and S46 respectively:The average genetic distances of BC ranged from 0.04 to 0.13 with a mean of 0.075.And the average genetic distances of RBC ranged from 0.02 to 0.35 with a mean of 0.139.The average genetic distances estimated by Lynch' s index of BC population were significantly lower than those of RBC population.Whereas the average genetic distances between BC and RBC ranged from 0.06 to 0.37 with a mean of 0.123.The mean number of alleles per microsatellite locus(1.89) in BC population was considerably lower than that(2.44) in RBC population. The average observed(0.34) and expected heterozygosity(0.39) in BC population were lower than those(0.54 and 0.51,respectively) in RBC population,indicating that the RBC presented higher genetic diversity than the BC.The results from RAP1) assay and microsatellite analysis were in agreement with each other,that is to say,the BC presented lower level of polymorphism than RBC.In addition,the RAPD and SSR analysis of genetic diversity of two kinds of bighead carp,provided scientific evidence and more information on the genetic background for bighead carp germplasm protection.