[Mechanism of apoptosis induced by specific COX-2 inhibitor SC236 in gastric cancer cells].
Zhonghua zhong liu za zhi [Chinese journal of oncology](2005)
摘要
OBJECTIVE:To investigate the underlying mechanism of apoptosis-inducing effect of a specific COX-2 inhibitor SC236 in gastric cancer cells.
METHODS:Western blot analysis was used to measure apoptosis-related proteins, cytochrome c, and caspase-3. The catalytic activity of the caspases was measured using a colorimetric assay.
RESULTS:Treatment of AGS gastric cancer cells with SC236 caused a significant elevation of the pro-apoptotic protein Bak, release of cytochrome c to the cytosol, and activation of caspase-3. A specific caspase-3 inhibitor, z-DEVD-fmk, blocked SC236-induced apoptosis.
CONCLUSION:SC236 inhibits cell growth and induces apoptosis in gastric cancer cells at least partly through the up-regulation of Bak, stimulation of cytochrome c release, and activation of caspase-3.
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关键词
apoptosis,stomach neoplasms,caspases,bak,cyclooxygenase-2
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