TUNEL determination of apoptosis effects induced by caffeic acid Ge on rat cervical carcinoma U14

Objective To use situ terminal deoxylnucleotidyl transferase mediated-UTP nick end labeling(TUNEL)for detecting the apoptosis effect induced by caffeic acid Ge on rat cervical carcinoma U14.Methods To establish the rat model of transplantable cervical carcinoma U14.The tumor inhibition rate was observed and the positive rate of apoptosis in various groups of tumor cells was detected by TUNEL.Results Caffeic acid Ge had the significant inhibitory effect on rats.The positive rate of apoptosis in high,middle and low dose groups were 45.47%,57.86% and 55.83%,respectively(P0.01).The TUNEL method showed that the positive rate of apoptosis in low dose group was the lowest,reaching 80.00% with statistical difference cpmpared with the contol group(P0.01).Conclusion Caffeic acid Ge has the significant inhibitory effect on rats cervical carcinoma U14 and induces the apoptosis of transplantable tumor U14,which could be the one of suppression tumor mechanisms