Quantitative Anti-F1 And Anti-V Igg Elisas As Serological Correlates Of Protection Against Plague In Female Swiss Webster Mice

A recombinant fusion protein composed of Yersinia pestis fraction I capsule (F1) and virulence-associated V antigen (V) (F1-V) has been developed as the next-generation vaccine against plague In this study, female Swiss Webster mice received a single intramuscular vaccination with one of eight doses of the F1-V vaccine and exposed 4 weeks later, to either Y pestis CO92 or C12 organisms by the subcutaneous or aerosol routes of infection Quantitative anti-F1 and anti-V immunoglobulin G (IgG) ELISAs were used to examine the relationship between survival outcome and antibody titers to F1 and V. Results suggested that each 1 log(10) increase in week 4 quantitative anti-F1 and anti-V IgG ELISA titers were associated with a I 7-fold (p = 0 0051) and 2 5-fold (p = 0 0054) increase in odds of survival, respectively. against either bubonic or pneumonic plague and may serve as serological correlates of protection Published by Elsevier Ltd