Effects of ginsenosides Rg1 on the intracellular free calcium concentration in primary cultured rat hippocampal neurons subjected to morphine-dependence.

Journal of Tropical Medicine(2009)

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摘要
Objective To investigate the effects of ginsenosides Rg1 on [Ca2+]i in primary cultured rat hippocampal neurons subjected to morphine-dependence.Methods After the hippocampal neurons subjected to morphine-dependence were respectively treated with ginsenosides Rg1(1 μmol / L,10 μmol/L and 100 μmol/L).Changes of [Ca2+]i induced by ginsenosides Rg1 were detected by laser scanning confocal microscope,by use of Fluo-3 / AM as a calcium fluorescent probe.Results [Ca2+]i in normal hippocampal neurons didn't have any significant changes after treated with low-,medium-and high-dose ginsenosides Rg1(P 0.05),but morphine induced a significant increase of [Ca2+]i in hippocampal neurons(P 0.01).In addition,low-,medium-and high-dose ginsenosides Rg1 could significantly block the increase of [Ca2+]i induced by morphine in a dose-dependent manner(P0.01)。 Conclusion The results indicated that ginsenosides Rg1 may effectively block the increase of [Ca2+]i induced by morphine and morphine addiction,and supported that ginseng can be a safe detoxification drug.
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morphine,hippocampal neurons,ginsensides,rg1,cell cultures,calcium
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