[Establishment of NCAM L1 minigene model and its splicing patterns in different cell lines].

OBJECTIVE:To establish a minigene model of neural cell adhesion molecule L1 (NCAM L1) gene and to study its splicing patterns in different cell lines. METHODS:Using human genetic cDNA as template, the NCAM L1 minigene fragment was amplified and inserted into eukaryotic expression vector. The minigene was transfected into 4 cell lines and the splicing patterns of NCAM L1 minigene in these cell lines were studied. RESULTS:The splicing patterns of NCAM L1 minigene were different in individual cell lines. In PFSK and Hela cell lines, two splicied isoforms were generated but in COS-1 and R28 cell lines, only one isoform existed. CONCLUSION:NCAM L1 minigene model can be used in alternative splicing analysis.