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兔抗PEB1多克隆抗体的制备及鉴定

Mei-na HOU,Xiao-ya GUO,Ting XIE,Yi QIU,Jie-ran SHI

Science Technology and Engineering（2012）

Cited 0|Views60
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Abstract
利用纯化的空肠弯曲菌表面蛋白PEB1,制备其多克隆抗体.用PEB1蛋白作为抗原免疫兔,分离血清得到兔抗PEB1多克隆抗体,用ELISA法检测抗体效价,并用Western-Blotting验证.PEB1蛋白免疫兔后,得到了兔抗PEB1多克隆抗体,间接ELASA法检测表明兔抗PEB 1血清效价达到1∶10 000,Western印记检测表明,该抗体能与PEB1蛋白发生特异性结合,其特异性与敏感性均较好.获得了高效价的PEB1多克隆抗体.
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Key words
campylobacter jejuni PEB1 polyclonal antibody
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