Effect of different influential factors on induction and multiplication of Paeonia suffruticosa buds in vitro

The axillary buds of Paeonia suffruticosa were selected as explants to study the effects of cultivars,basic mediums,time of axillary buds collection,dark treatment,medium types and mass concentration of plant growth regulators and subculture cycle on induction and multiplication of Paeonia suffruticosa axillary bud in vitro.The results showed that cultivars and collection time of axillary buds were the main factors affecting its induction,January and February was the suitable collection time,but GA_3 mass concentrations,dark treatment and basic mediums had less influence on axillary buds induction.In the four basic mediums,the medium with MS+Ca2+ was the best one for plantlet multiplication,the medium with 6-BA and IAA had some influence on plantlet multiplication coefficient,the best multiplication mediums was MS+Ca2++6-BA 2.0 mg·L-1+IAA 0.3 mg·L-1,and the suitable subculture cycle was 35 d for plantlet multiplication.