Identification of the Superiority Strains in the Biological Desulfurization Reactor by PCR-DGGE Melhodology Treating High Salinity Wastewater

In order to identify the superiority strains in the biological desulfurization reactor treating high salinity wastewater,total microbial DNA was extracted from sludge samples of bio-desulfurization bioreactor operated continuously for 1 year.16S rRNA fragments were amplified with the universal primers(GC341F/534R),and amplified DNA fragments were separated by denaturing gradient gel electrophoresis(DGGE).The DGGE bands in the gel were excided for sequencing.Homology analysis was conducted by comparing these gene sequences based on GenBank database.Results showed that the superiority strains were Thiobacillus sp.ISA11,Halothiobacillus sp.ST15,Marine bacterium HB-5,Uncultured bacterium clone J69-151C-2A,among which Thiobacillus sp.ISA11 and Halothiobacillus sp.ST15 were the major desulfurizing strains.