Quantitative fluorescent PCR to verify critical value specimens flow oytometry method determination of human leukocyte antigen B27 livability

OBJECTIVE To observe the critical value to verify the specimens flow cytometry(FCM) detection of human leukocyte antigen expression with the real-time fluorescence quantitative polymerase chain reaction(PCR) method and assess its value in the clinical diagnosis and treatment of ankylosing spondylitis(AS).METHODS A total of 482 patients with flow cytometry detection of human leukocyte antigen expression were positive,the critical value,negative samples and 100 samples of healthy people,the peripheral blood of HLA-B27 DNA were detected by quantitative real-time fluorescence PCR to determine the positive rate of samples.RESULTS There were 282 cases tested in the positive group with the PCR with the positive rate of 100.00%,there were 80 cases tested in the critical value group with the positive rate of 91.25%,and there were 120 cases tested in the negative group with the positive rate of 0.83%.Of 100 cases investigated in the control group,the positive rate was 3.00%.CONCLUSION With the quantitative fluorescent PCR detection higher than 103 as the standard,the positive rate of FCM threshold is too high,the difference is statistically significant,and there is a false positive case;there is no significant difference between the negative group and the positive group,but there is false negative case in the negative group.The quantitative PCR has significant value in the diagnosis of convection cytometry detection of specimens of the critical value so as to prevent the false positive case and false negative case,increasing the diagnosis rate of AS and the reliability.