An investigation for the effect of HLA-DRB1 * 07, 13 allelic genes on the immune response to hepatitis B vaccination.

Objective:To investigate the effect of HLA-DRB1*07,13 allelic genes on the immune response to hepatitis B vaccination.Methods:896 healthy college students of Han nationality from Guangxi province,who had received standard courses of vaccination with recombinant hepatitis B virus vaccine,were tested the level of anti-HBs with ELISA method at the sixth month after their last vaccination.The non-or hypo-responders were selected to receive another 20 μg doses of recombinant vaccine and were examined anti-HBs once more four weeks later.99 non-or hypo-responders and 136 medium or hyper-responders were selected for the study subjects.HLA-DRB1*07,13 allelic genes of the subjects were detected by polymerase chain reaction using sequence specific primer(PCR-SSP) method.Results:The frequencies of HLA-DRB1*07 gene in the non-or hypo-responders group were significantly higher than that in the medium or hyper-responder group(16.16% vs 4.41%,P<0.05). No significant difference of HLA-DRB1*13 gene between the two groups(1.01% vs 3.68%,P=0.389).Conclusion:There is a close relationship between the expression of HLA-DRB1*07 gene and the non-or hypo-response to HB vaccine;No relationship between HLA-DRB1*13 gene expression and the response to HB vaccine is found.