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流动注射化学发光法测定强力霉素

WANG Hao,WANG Rui-fen,HUANG Yu-ming

Journal of Southwest University(Natural Science Edition)（2007）

Cited 9|Views1
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Abstract
HNO3酸性介质中,强力霉素与Ce(Ⅳ)反应产生化学发光,罗丹明6G对该反应有较强的增敏作用.据此,采用流动注射技术建立了简易、快速测定盐酸强力霉素的化学发光新方法.在优化的实验条件下,化学发光强度在1·0×10-8～1·0×10-6g/mL范围内与强力霉素的浓度呈现良好的线性关系.根据IUPAC的建议计算得到的检出限为2×10-9g/mL(3σ),对1·0×10-6g/mL的强力霉素进行了11次平行测定,其相对标准偏差为1·5%.将本法用于强力霉素药片及血样和尿样中强力霉素的测定,结果令人满意.
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Key words
cerium(Ⅳ),chemiluminescence,Rh6G,doxycycline
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