高分离度快速液相色谱法测定小儿清热利肺口服液中绿原酸和牛蒡苷的含量
China Pharmacy(2012)
Abstract
目的:建立同时测定小儿清热利肺口服液中绿原酸和牛蒡苷含量的方法。方法:采用高分离度快速液相色谱法。色谱柱为ZorbaxSB-C18柱,流动相为0.1%冰醋酸溶液-乙腈(梯度洗脱),检测波长0~5.5min为327nm,5.5~7.0min为280nm,流速为1.0mL.min-1,柱温为20℃,进样量为1.0μL,采集频率为1s。结果:绿原酸、牛蒡苷进样量分别在0.014602~0.29204、0.051396~1.02792μg范围内与峰面积积分值有良好的线性关系(均r=0.9999);平均加样回收率分别为99.56%、99.96%,RSD分别为1.22%、1.09%(n=6)。结论:本方法操作简便易行,可在保持分离度的前提下缩短分离时间,减少溶剂消耗,节约分析成本,提高日常检验工作的效率,可用于控制该药的生产工艺及质量。
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Key words
Arctiin,Chlorogenic acid,Xiao'er qingre lifei oral solution,Content determination,Rapid resolution liquid chromatography
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