人结直肠癌细胞AnnexinA2基因敲除细胞系的建立
Journal of Shaanxi Normal University(Natural Science Edition)(2012)
Abstract
为研究ANXA2与结直肠癌发展的关系以及其在肿瘤发展过程中作用的构效关系,构建了ANXA2基因敲除质粒重组子,然后导入人结直肠癌细胞系Cac02,经过一系列筛选过程,最终建立了基因敲除ANXA2的Cac02细胞系(ANXA2-/-Cac02).经过PCR和WesternBlotting检测,确认该细胞系无任何野生ANXA2表达.
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Key words
Caco2 cell,Annexin A2,gene knockout
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