芦荟叶片的取材处理对愈伤组织诱导的影响
Anhui Agricultural Science Bulletin(2012)
Abstract
以中华芦荟叶片为外植体,研究不同取材方式和不同激素配比对愈伤组织诱导的影响。实验结果表明,叶片中上部外植体优于叶基部外植体,叶片中上部外植体的3种取材方式中,带有少量叶肉细胞的表皮细胞层是愈伤组织诱导的最佳外植体;MS+BA 3.5mg/L+NAA 1.0 mg/L+2,4-D 0.2 mg/L+KT 0.5 mg/L是最适的愈伤组织诱导培养基,其愈伤组织诱导率达91.7%,且愈伤组织生长良好。
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Key words
Aloe vera L.var.chinensis,Plant hormone,Callus induction,Leaf
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