新生大鼠心肌细胞原代培养方法的改进

Journal of Hebei Normal University(Natural Science Edition)(2007)

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Abstract
探讨更为简单的SD大鼠心肌细胞原代培养的方法,使分离的心肌细胞达到理想的存活率和纯度,为临床应用建立心肌细胞模型.取出生24 h内SD大鼠的左心室,剪碎、消化、分离和纯化,进行培养,0.1 g/L胰酶消化,可以分离到形态完整、贴壁生长的心肌细胞.进一步通过离心、差速贴壁和化学试剂抑制非心肌细胞生长,纯化得到95%以上的心肌细胞.成功建立了心肌细胞体外培养模型,获得了高纯度的心肌细胞.
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Key words
primary culture,cardiac myocytes,new natal rats
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