Bead-Based Approaches to Develop Thioaptamers for Diagnostics and Therapeutics

In this presentation, we will outline our novel, integrated bead-based oligonucleotide selection procedures including the automation of the split-pool synthesis of a one-bead one-thioaptamer library, high-throughput flow cytometric sorting and a PCR-based sequencing. Additionally, we will also discuss one promising thioaptamer that has been shown in preliminary animal therapeutic dosing to increase survival in animal models of infection with West Nile virus. Moreover; we will present our ongoing work on a thioaptamer proteomics chip.