Transcriptional Regulation Of Monocyte Chemotactic Protein-1 Release By Endothelin-1 In Human Airway Smooth Muscle Cells Involves Nf-Kappa B And Ap-1

Endothelin-1 (ET-1) is implicated in airway inflammation in asthma, but the mechanisms of its effects are poorly understood. We studied the effect of ET-1 on expression of the chemokine, monocyte chemotactic protein-1 (MCP-1), in primary cultures of human airway smooth muscle cells.MCP-1 release was measured by elisa. Pharmacological antagonists/inhibitors, reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting were used to study ET receptors and kinase cascades. Transcriptional regulation was studied by real-time RT-PCR, transient transfection studies and chromatin immunoprecipitation assay. Major findings were confirmed in cells from three donors and mechanistic studies in cells from one donor.ET-1 increased MCP-1 release through an ETA and ETB receptor-dependent mechanism. ET-1 increased MCP-1 mRNA levels but not mRNA stability suggesting it was acting transcriptionally. ET-1 increased the activity of an MCP-1 promoter-reporter construct. Serial deletions of the MCP-1 promoter mapped ET-1 effects to a region between -213 and -128 base pairs upstream of the translation start codon, containing consensus sequences for activator protein-1 (AP-1) and nuclear factor-kappa B (NF-kappa B). ET-1 promoted binding of AP-1 c-Jun subunit and NF-kappa B p65 subunit to the MCP-1 promoter. Blocking the inhibitor of kappa B kinase-2 with 2-[(aminocarbonyl)amino]-5-[4-fluorophenyl]-3-thiophenecarboxamide (TPCA-1) decreased ET-1-stimulated MCP-1 production. p38 and p44/p42 mitogen-activated protein kinases were involved in upstream signalling.ET-1 regulated MCP-1 transcriptionally, via NF-kappa B and AP-1. The upstream signalling involved ETA, ETB receptors, p38 and p44/p42 mitogen-activated protein kinases. These may be targets for novel asthma therapies.