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德化黑鸡黑素皮质素受体1(MCIR)基因单核苷酸多态性分析

Nen-zhu ZHENG,Xiao-ning DONG,Hui CHEN,Li-zhen ZHENG,Zhi-ming ZHU,Zhong-wei MIAO,Hong-tao LIU,Yu-qiong LAI,Jian-zheng ZENG,Long-xing CHEN

Fujian Journal of Agricultural Sciences（2008）

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Abstract
采用聚合酶链式反应及单链构象多态(PCR-SSCP)技术以及DNA直接测序的方法,对德化黑鸡MCIR基因编码区进行单核苷酸多态性(SNPs)分析.结果表明:引物Pz扩增片段具有多态性,并表现为AA、AB和BB等3种基因型;经序列分析发现,BB型存在2个突变位点:分别在1 094处(G-A)和1095处(T-C),其中在1 095处的突变还导致氨基酸的改变(Cys-Arg).由基因型分布情况表明,德化黑鸡C系(乌色)和D系(非乌色)均以AA型为主;BB型(含SNP)仅存在于C系中,而在D系中未发现,推测BB型可能是乌色性状特有或与乌色性状相关.
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Key words
single nucleotide polymorphism,MC1R gene,Dehua Black Chicken
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