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中波紫外线对HaCaT细胞增殖活性的影响

Chinese Journal of Aesthetic Medicine(2011)

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Abstract
目的:探讨中波紫外线(UVB)对HaCaT细胞损伤的影响。方法:取对数生长期的HaCaT细胞,用0、30、60、90 mJ/cm2剂量的UVB照射后继续培养0、24、48、72h,在倒置显微镜下观察其形态学变化,用MTT法检测UVB照射对细胞增殖活性的影响。结果:在30~60mJ/cm2的UVB辐射下,随辐射剂量的增大,细胞的增值活性逐渐下降,而细胞的凋亡率逐渐增加,倒置相差显微镜从形态学上证实了细胞的凋亡改变。结论:UVB可诱导细胞凋亡,且呈剂量依赖性。
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Key words
irradiation,HaCaT cells,UVB,damadge
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