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响应面分析法优化金线莲多糖提取过程的研究

YU Jie,CUI Shi-chao,ZHANG Xiao-hui,GUO Mei-ru

Journal of Shantou University(Natural Science Edition)（2009）

Cited 5|Views7
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Abstract
以经乙醇提取黄酮后的金线莲滤渣为材料,利用响应面法优化多糖的提取过程.在单因素实验的基础上,分别选定液料比、提取时间和温度3个水平进行响应面实验,建立金线莲多糖提取率的二次回归方程,通过回归分析及岭脊分析得到优化组合条件.研究结果表明,当提取过程中液料比为35∶1,提取时间和温度分别为2.2h和83℃时,金线莲多糖提取率的理论预测值为3.42%,验证值为3.47%±0.47%,两者相差不大,提取条件参数可靠.
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Key words
response surface analysis,polysaccharides,Anoectochilus roxburghii
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