Kinetic Delay Of Cyclization/Elimination-Coupled Enzyme Assays: Analysis And Solution

A kinetic analysis of an enzyme assay employing a synthetic substrate that produces a detectable signal through a spontaneous organic cyclization/elimination reaction following the enzymatic reaction was conducted. The results from the calculation were used to predict the lag period and provide accurate measurements of the activity of alkaline phosphatase using the fluorogenic substrate ( 1). (C) 2010 Published by Elsevier Ltd.