[Analysis of the kinematic parameters of hyperactivated Guinea pig spermatozoa].

OBJECTIVE:To evaluate the role of sperm kinematic parameters in the hyperactivation of Guinea pig spermatozoa, and to confirm the index of their hyperactivated motility. METHODS:Computer-aided sperm analysis (CASA) was used to describe the kinesis parameters of the Guinea pig spermatozoa incubated for 1, 3, 5 and 7 hours. RESULTS:The curvilinear velocity, average path velocity and amplitude of lateral head displacement were increased with time and reached the peak at 5 hours, while the straight linear velocity, linearity, straightness and beat cross frequency were gradually decreased with time and hit the bottom at 5 hours. CONCLUSION:The sperm movement pattern changes greatly before hyperactivation during the capacitation of Guinea pig spermatozoa.