Effects of High Protein Diet on the Pharmacokinetics of Levofloxacin in Dogs

OBJECTIVE:To study the effects of high protein diet on the pharmacokinetics of levofloxacin in dogs.METHODS:6 dogs were randomly divided into fasting group and high protein diet group;high protein diet group received high protein diet 150 g in each;both groups were given levofloxacin 10 mg/kg.The blood concentration of levofloxacin in dogs was determined by HPLC 0.5,1,2,4,8,12,24 and 48 h after medication,randomly crossed experiments were carried out 2 weeks later;and pharmacokinetic parameters were calculated with 3p97 software.RESULTS:Pharmacokinetics process of Levofloxacin in 2 groups dogs were in accordance with the two compartment model.Main pharmacokinetic parameters of fasting group vs.protein group were as follows:t 1/2 β:(11.07±8.46)h vs.(21.65±4.65)h;t max:(3.42±0.41)h vs.(1.53±0.34)h;c max:(2.71±0.60)μg/ml vs.(2.22±0.22)μg/ml;AUC 0-48 h:(36.51±8.66)μg · h/ml vs(.29.78±4.26)μg · h/ml,respectively.There was significant difference in t max(P0.01),while other parameters had no significant difference between 2 groups(P0.05).CONCLUSIONS:High protein diet can reduce t max,promote absorption of levofloxacin.