Changes of phytohormone contents during anther abortion of male sterile new grapevine selection ‘2004-6-12’

'2004-6-12' is a male sterile grape line selected from the self cross progeny of the cultivar 'Wink' which belongs to the species of Vitis vinifera by Nanjing Agricultural University.ELISA(enzyme-linked immunosorbent assay) was used to detect the contents of IAA(indole acetic acid),GAs(gibberellic acids),CTK(cytokinin)and ABA(abscisic acid) at different developmental stages of anther,including sporogenous stage,microspore mother cell stage,meiosis stage,tetrads stage,mononuclear microspore stage,binuclear stage and mature stage.The results showed that the differences in the contents of IAA and GAS in the floral buds were not significant between '2004-6-12' and 'Wink' before meiosis,but the contents were much lower in '2004-6-12' than in'Wink' at the stages of tetrad and uninucleate.The content of ABA began to decrease at the stage of mononuclear microspore in 'Wink',while at binuclear stage in '2004-6-12'.The ratios of IAA /ABA,GAs/ABA were remarkably higher in '2004-6-12' than in 'Wink' before binuclear stage.The results suggested that the deficiency of IAA,GAs and CTK,the delayed reduction of ABA and the imbalance of the ratios of the IAA /ABA,GAs/ABA contributed to the male sterility of grape.