Optimization of Preparation Conditions for Soybean Anticancer Peptides by Papain Hydrolysis

In this study,operating conditions for the enzymatic preparation of anticancer peptides from soybean protein isolate with papain were optimized for maximum inhibitory rate against the growth of human gastric cancer SGC-7901 cells.Peptides with the highest anticancer activity were obtained after 4 h of hydrolysis at pH 7.5 and 55 ℃ with papain at a dose of 7000 U/g and a substrate concentration of 6 g/100 mL.All hydrolysates collected at selected time points during the hydrolysis process had obvious anticancer activity,while no linear relationship between degree of hydrolysis and cell growth inhibitory rate was found.All substrate concentration,enzyme dose,hydrolysis temperature,hydrolysis time and pH could affect cell growth inhibition rate of hydrolysates.