Avidin–Biotin Assembling of Horseradish Peroxidase Multi-Monomolecular Layers on Electrodes
AUSTRALIAN JOURNAL OF CHEMISTRY（2006）
摘要
The strong avidin - biotin affinity is used to stack up successive monomolecular layers of horseradish peroxidase on carbon electrodes. After a biotinylated immunoglobulin is adsorbed on the electrode surface, alternate deposition of neutravidin and biotinylated HRP allows the assemblage of up to 16 successive active HRP layers. The film buildup is followed by cyclic voltammetry using an osmium complex as soluble mediator and H2O2 as substrate. The variation of the resulting catalytic responses with H2O2 concentration exhibit characteristics qualitatively consistent with the catalysis-inhibition reaction scheme previously established for monomolecular layers. In most cases the catalytic activity increases steadily with the number of monomolecular layers, leading to a significant increase of the analytical sensitivity of the derivatized electrode.
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关键词
biological chemistry,educational,density functional theory,computational chemistry,catalysis,reaction mechanisms,organic chemistry,electrochemistry,colloids,pharmaceutical chemistry,mass spectrometry,biocatalysis,nanotechnology,surface chemistry,crystal structures,medicinal chemistry,supramolecular chemistry,self assembly,inorganic chemistry,peptide,combinatorial chemistry,kinetics,photochemistry,proteins,physical chemistry,sensors,combinatorial,crystallography,quantum chemistry,enzymes,green chemistry,ab initio calculations,amino acids,spectroscopy,macromolecules,structure,polymer chemistry,interfaces,analytical chemistry,biosensors,ionic liquids
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