The changes of macrophages and dendritic cells in intima between upstream and downstream of abdominal aorta constriction in atherosclerosis rabbits.

Objective To observe the changes of macrophages and dendritic cells in intima between upstream and downstream of abdominal aorta constriction in atherosclerotic rabbits.Methods We divided New Zealand rabbits into common diet group and high-lipid diet group.After 28 weeks,we referred to them different operations,then divided them into 3 groups,they were high-lipid operation group(Ho group)、common control group(Cc group) and high-lipid control group(Hc group).After operations,we continued to keep them for 4 weeks,then measured blood pressure in different positions of their abdominal aortas and took them.Anti-CD68 and s-100 antibodies were used to identify macrophages and dendritic cells.Results(1)TG of High-lipid group(contains Ho and Hc group) was 4.71±0.73 mmol/L,and TC was 22.82±3.01 mmol/L;TG of common control group was 1.42±0.24 mmol/L,and TC was 1.96±0.19 mmol/L.(2)There was an obvious difference of blood pressure(BP) between upstream and downstream of constriction in Ho group.BP in upstream of constriction was higher in Ho group than it in upstream of abdominal aorta in Hc group,and lower in downstream of constriction than it in downstream of abdominal aorta in Hc group.(3)Cc group:The intima was smooth and glossy;Hc group:there were a lot of foam cells in the intima;Ho group:the intima was thicker in upstream of constriction than it in upstream of abdominal aorta in Hc group,and thinner in downstream of constriction than it in downstream of abdominal aorta in Hc group.4,Macrophages and dendritic cells were more in upstream of constriction in Ho group than them in upstream of abdominal aorta of Hc group,and less in downstream of constriction than them in downstream of Hc group.Conclusions The blood pressure was higher in upstream of constriction,and the intima was thicker,macrophages and dendritic cells were more;and all the above were less in downstream of constriction.