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应用均匀设计优化香石竹组培苗增殖培养

Jing-run XIU,Xuan-chun PIAO,Mei-lan LIAN,Chang-chun LIN

Journal of Agricultural Science Yanbian University（2008）

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Abstract
应用均匀设计方法对香石竹组培苗的增殖体系进行优化研究,设计BA浓度、琼脂用量、蔗糖浓度、通气孔大小和氨态氮浓度5个因素试验,对观察值进行方差分析,结果表明:MS+BA 0.1 mg/L+琼脂7.5 g/L+蔗糖35 g/L+铵态氮10 mM处理相对较好,通气口大小2.25 cm,通过10个处理的回归分析得出5个因素对香石竹试管苗生长作用中不同调查项目4个拟合回归方程,并计算出极值和最佳因素配比,发现BA不适宜用在香石竹的增殖培养.
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Key words
uniform designing,Dianthus caryophyllus L.,tissue culture
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