Experiment on Multidrug-Resistance Reversing Effect of Puerarin Injection in Patients with Gastric Carcinoma

Objective To investigate the effect of puerarin injection on human drug-resistant cell line SGC7901/VCR.Methods The drug sensitivity test was carried out with WTT assay,the change of cell cycle and concentration of drug in cytoplasma was measured by flow cytometric method,the distribution of muhidrug-resistance relevant protein(MRP)and P-glucoprotein(PGP)in drug-resisent cells were determined by immunohistochemical stainning,and the tumor inhibitory rate of puerarin and chemotherapeutic agents,including 5-FU,VCR,DDP and ADM,in nude rats with transplanted gastric carcinoma in situ was observed.Results Puerarin injection could re- verse the drug resistance of SGC7901/VCR to 5-FU,the proportion of cells in S phase and G_2 plus M phase in- creased,and that in G_1 phase decreased as compared to the untreated control.In the same time,the fluorescent in- tensity of adriamycin in cells was significantly increased,the expression of MRP and P-GP were significantly de- creased.The tumor inhibitory rate in nude rats with gastric carcinoma treated by combined puerarin and 5-FU was higher than that only treated with 5-FU alone.Conclusion Puerarin injection could reverse the multidrug resistance of SGC7901/VCR,its mechanism may relate with its action in decreasing protein expression of MRP and P-GP.