人胃癌细胞系HGC-27肿瘤球细胞干样特性研究

Journal of Modern Oncology(2012)

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Abstract
目的:探讨体外培养条件下人胃癌细胞系HGC-27肿瘤球细胞的干样特性。方法:SFM培养人胃癌细胞系HGC-27的肿瘤球,细胞计数绘制HGC-27细胞和肿瘤球细胞的生长曲线,96孔培养板接种计算细胞的克隆形成能力。结果:成功培养出HGC-27细胞的肿瘤球。生长曲线和克隆形成实验显示,肿瘤球细胞的增殖和克隆形成能力高于HGC-27细胞。结论:HGC-27细胞可以培养成肿瘤球细胞,肿瘤球细胞比HGC-27细胞更具有干样特性。
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Key words
gastric cancer,HGC-27 cell line,tumor sphere cell,like-stem characteristic
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