Serological survey of canine leptospirosis in the tropics of Yucatan Mexico using two different tests

Blood samples were taken from 400 stray dogs. The microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA) test were implemented using Leptospira interrogans serogroups canicola, hardjo, pyrogenes, panama, pomona, tarassovi, icterohaemorrhagiae, gryppotyphosa, wolffi and brastislava. For the ELISA test, sonicated antigen from above mentioned cultures was used. A conjugate AP-labeled anti-dog IgG antibody was used, the optimal cut-off point of ELISA was set at 1.34. Concordance between ELISA and MAT titers was measured by kappa (κ).