Determination Of Xylitol And Sorbitol In Sugar-Free Chewing Gums By Miniaturized Capillary Electrophoresis System With Amperometric Detection

A miniaturized capillary electrophoresis with electrochemical detection (CE-ECD) system was developed for the separation and determination of xylitol and sorbitol in three commercial sugar-free gums. Factors influencing the separation and detection processes were examined and optimized. These two analytes have been separated within 10 min at a separation voltage of 4 kV in 70 mmol/L NaOH running buffer. Linear response was obtained at the range of 5.0 x 10(-5) -1 x 10(-2) mol/L and 5.0 x 10(-5) -5.0 x 10(-2) mol/L with the detection limits (S/N = 3) of 5.0 x 10(-6) mol/L and 2.5 x 10(-6) mol/L for xylitol and sorbitol, respectively (r > 0.9997). The proposed method was successfully applied to determine the gum samples with the RSD and average recoveries of 3.7%, 4.5% and 98.1%, 91.1% for xylitol and sorbitol, respectively (n = 10).