[Determination of tomatine in Solanum cathayanum by RP-HPLC].

OBJECTIVE:To develop an HPLC method for determination of tomatine in the dried Solanum cathayanum of China Hubei Enshi. METHOD:The analysis was performed on a YMC-Pack ODS-AA column (4. 6 mm x 150 mm, 5 microm) eluted with acetonitrile and water in gradient mode. The concentration of acetonitrile in the mobile phase changes from 20% to 100% within 60 minutes. The detection wavelength was set 203 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C. RESULT:The linear relationship of tomatine was determined within the range from 0.1-0.6 g x L(-1) (r = 0.999 7). The average recovery as 98.93% with RSD 1.2%. CONCLUSION:A convenient and reliable method was developed to determine the content of tomatine in the dried S. cathayanum.