奶牛载脂蛋白B100基因克隆及原核表达
China Animal Husbandry & Veterinary Medicine(2012)
Abstract
本研究根据GenBank已收录奶牛ApoB100基因序列,设计特异性引物获得目的基因。经pGM-T载体克隆,对重组质粒进行BamHⅠ和EcoRⅠ双酶切鉴定并测序,序列同源性达100%;将目的基因连接到pET-28a表达载体中,提取质粒,转化到Rosetta(DE3)中,筛选的阳性克隆经IPTG诱导。SDS-PAGE初步分析表明,成功获得分子质量为35ku的蛋白质;Western blotting结果呈阳性,表明通过本试验成功获得了目的蛋白。
MoreTranslated text
Key words
prokaryotic expression,ApoB100,gene cloning
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined