RP-HPLC法测定咽炎片中哈巴俄苷、肉桂酸、丹皮酚、芸香苷、黄芩苷的含量
Chinese Journal of Pharmaceutical Analysis(2009)
Abstract
目的:建立高效液相色谱法同时测定咽炎片中哈巴俄苷、肉桂酸、丹皮酚、芸香苷和黄芩苷5种有效成分的含量。方法:采用Kromacil C18柱(4.6mm×200mm,5μm),以甲醇-0.05%磷酸水溶液为流动相梯度洗脱,流速1mL·min-1,于278nm波长下检测,柱温为35℃。结果:哈巴俄苷、肉桂酸、丹皮酚、芸香苷和黄芩苷进样浓度分别在0.200~4.00,0.208~4.16,0.610~12.2,0.960~19.2,1.00~20.0μg·mL-1范围内线性关系良好;平均回收率(n=6)分别为100%,102%,101%,98.3%,101%。结论:本方法简便,准确可靠,重现性好,能有效测定该药品主要成分的含量,具有较强的实用价值。
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Key words
baicalin,cinnamic acid,Yanyan tablets,rutoside,harpagoside,RP-HPLC,paeonol
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