外源产甲烷菌降解褐煤产气实验

Petroleum Exploration and Development(2012)

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Abstract
为研究外源菌降解褐煤产气能力,从厌氧消解污泥中提取产甲烷菌群,以褐煤为碳源进行菌种驯化,开展产气实验,并对驯化后菌群的产气影响因素进行实验研究。驯化实验发现:驯化后该菌群初始产气时间由驯化前的12 d缩短为6 d,且30d内产气总量增加了29.2%;经检测,生成气体主要为CH4和少量CO2,未检测到重烃气。产气影响因素实验表明:该菌群最佳产气初始培养基pH值为7.0,产气量分别约为初始pH值为6.4、7.4时的1.9倍和2.4倍;煤的粒径也是影响产气量的因素之一,表现为粒径越小,产气量越大,但产气量随粒径变小变化不明显;培养基本身产气量约占总产气量的50%,原因可能是其中添加了微量L-半胱氨酸(0.5g/L)和酵母浸出液(1 g/L)。
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Key words
methanogen,brown coal,exogenous bacteria,domestication,coal-derived gas
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