高效液相色谱法测定三维樟柳碱膜中氢溴酸樟柳碱含量
China Pharmaceuticals(2013)
Abstract
目的建立测定三维樟柳碱膜中氢溴酸樟柳碱含量的高效液相色谱法。方法采用Waters C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-pH=3.5的0.02 mol/L磷酸二氢钾溶液(7∶93),流速为1.0 mL/min,检测波长为210 nm,进样量为100μL。结果樟柳碱质量浓度在0.799~7.990μg/mL范围内与峰面积呈良好的线性关系,r=0.999 9(n=6),平均回收率为99.95%,RSD=1.38%(n=9)。结论所用方法简单、专属性强,可用于三维樟柳碱膜中氢溴酸樟柳碱含量的测定。
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Key words
HPLC,anisodine hydrobromide,leukoplakia pellicles
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