离体毛囊切片两种制作方法的比较及凋亡检测
Journal of Xi'an Jiaotong University(Medical Sciences)（2006）
Abstract
目的对人头皮游离毛囊(HF)切片制作及凋亡检测方法进行比较.方法分别将石蜡切片法和冰冻切片法应用于游离毛囊;利用原位缺口末端标记法(TUNEL)进行荧光染色与3,3'-二氨基联苯胺(DAB)染色,检测未经培养的游离毛囊及培养5 d的游离毛囊的不同部位凋亡细胞情况.结果在对游离毛囊形态检测中,各组毛囊在外根鞘(ORS),毛球(HB)下部,真皮鞘(DS)及毛乳头(DP)中的凋亡细胞数并无显著性差异(P＞0.05).结论冰冻切片法应用于游离毛囊简单有效,TUNEL原位荧光结合DAB染色适合于检测毛囊细胞凋亡情况.
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Key words
Apoptosis,Culture in vitro,Hair follicle
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