Determination of Diosgenin Content in RHIZOMA PARIDIS with Different Growth Years by HPLC

[Objective] To discuss the relationship between diosgenin content in RHIZOMA PARIDIS and its growth years,so as to provide a reference basis for the optimal harvesting years of RHIZOMA PARIDIS.[Method] The diosgenin content was determined by HPLC,and the HPLC conditions were:chromatographic column of Agilent zorbax SB-C18 column（4.6 mm×150.0 mm,5 μm）,mobile phase of acetonitrile-water at the volume ratio of 90:10,flow rate of 1.0 ml/min,detection wavelength of 203 nm and column temperature of 35 ℃.[Result] Diosgenin content in different growth years RHIZOMA PARIDIS varied significantly.The diosgenin content was the highest in seven years old RHIZOMA PARIDIS,followed in the five and six years old ones,and the least in three and four years old ones.[Conclusion] RHIZOMA PARIDIS with different growth years varied from each other in diosgenin content,and should be harvested in terms of different aims in practical application.