A Complete System for Identifying Inhibitors of Creatine Kinase B
Analytical Biochemistry(2000)
Abstract
We have developed a complete system for discovery of lead compounds as inhibitors of creatine kinase B. In this article, we describe production and purification of the recombinant protein, conditions and features of an optimized high-throughput screening assay, and results of our implementation of the system using a diverse compound library.
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