Polarographic analysis for corticosteroids: Part 1. The electroanalytical properties of corticosteroids
Analytica Chimica Acta(1978)
Abstract
The electroanalytical behaviour of corticosteroids has been studied in different supporting electrolytes. The reduction patterns are established by examining pH profiles, by constant-potential coulometry, fast-sweep voltammetry, potentiometry with ion-selective electrodes and differential pulse polarography. Dimerization and alcohol formation take place with the reduction of the C-3 keto group resulting in one or two peaks, depending on the number of double bonds in the A-ring and the pH. The C-20 keto group is reduced to an alcohol. Both reduction steps can be used for analytical purposes. The differential pulse peak height is linear with the concentration down to 10-6 M steroid.
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Key words
polarographic analysis
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