Phospholipid activation of Lactobacillusplantarum undecaprenyl pyrophosphate synthetase

The ability of a number of phospholipids to stimulate Lactobacillusplantarum undecaprenyl pyrophosphate synthetase was investigated. The detergent Triton X-100, which is added to stabilize the enzyme during purification and is required for invitro activity, was removed with the non-ionic resin XAD-2. The effects of cardiolipin, phosphatidyl ethanolamine, phosphatidyl choline, and phosphatidyl glycerol on the activity of XAD-2 treated undecaprenyl pyrophosphate synthetase were determined. Of the phospholipids studied only cardiolipin stimulated invitro enzymic activity as effectively as Triton X-100.