聚酰胺柱层析法分离回心草总黄酮的研究
Journal of Pharmaceutical Practice(2009)
Abstract
目的:确定聚酰胺柱层析法分离回心草总黄酮的条件。方法:对聚酰胺用量、乙醇洗脱浓度、洗脱速度等方面进行考察。结果:干法上样,聚酰胺用量以药材:聚酰胺=1:1为佳,以50%的乙醇为洗脱溶剂,2倍柱体积/h为最佳的洗脱速率。结论:聚酰胺可较好地用于回心草总黄酮的分离富集。
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Key words
polyamide,total flavonoids,Rhodobryum giganteum(Schwaegr.) Par.
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