[Expression of the voltage-dependent anion channel gene in human ejaculated spermatozoa].
Zhonghua nan ke xue = National journal of andrology(2009)
摘要
OBJECTIVE:To identify the genes involved in sperm motility.
METHODS:We hybridized asthenospermia and normal motile sperm cDNA samples with the human whole genome Affymetrix chip to screen differentially expressed genes. Then we detected the mRNA expressions of the voltage-dependent anion channel genes (VDACs) in human organs and spermatozoa by RT-PCR and compared their expressions in the poor and normal motility spermatozoa.
RESULTS:Differentially expressed genes VDACs were identified by analysis of the hybridization signals, including the 3 subtypes VDAC1, VDAC2 and VDAC3. The expression of VDAC2 mRNA was significantly decreased in the poor motility sperm (0.568 +/- 0.036), as compared with the healthy men (0.803 +/- 0.043, P < 0.01).
CONCLUSION:The decreased expression of VDAC2 in the ejaculated spermatozoa is possibly associated with the reduction of sperm motility.
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关键词
gene chip,reverse transcription polymerase chain reaction,voltage-dependent anion channel,asthenospermia
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